RETINOIC ACID-INDUCED DIFFERENTIATED NEUROBLASTOMA-CELLS SHOW INCREASED EXPRESSION OF THE BETA-A4 AMYLOID GENE OF ALZHEIMERS-DISEASE AND AN ALTERED SPLICING PATTERN

被引：98

作者：

KONIG, G ^{[1
]}

MASTERS, CL ^{[1
]}

BEYREUTHER, K ^{[1
]}

机构：

[1] UNIV MELBOURNE,DEPT PATHOL,PARKVILLE,VIC 3052,AUSTRALIA

来源：

FEBS LETTERS | 1990年 / 269卷 / 02期

关键词：

Alternative splicing; Alzheimer's disease; Neuroblastoma cell line; Retinoic add; βA4 amyloid precursor protein;

D O I：

10.1016/0014-5793(90)81181-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Retinoic add (RA) induced differentiation of SH-SY5Y neuroblastoma cells is associated with more than a tenfold induction of total Alzheimer's disease βA4 amyloid protein precursor (APP) mRNA as analyzed by Northern blot hybridisation. Sl nuelease protection experiments reveal that the splicing pattern of these differentiated cells is altered in favor of APP695 mRNA, coding for the shortest amyloidogenic βA4 amyloid precursor protein. Induction of differentiation of SH-SY5Y cells with NGF leads to a fivefold increase of total APP mRNA without change in the splicing pattern. This suggests that RA but not NGF induces factor(s) which are responsible for an APP hnRNA splicing favoring APP695 mRNA. © 1990.

引用

页码：305 / 310

页数：6

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