RADIOIMMUNOLOGICAL SCREENING METHOD FOR SPECIFIC MEMBRANE-PROTEINS

被引：66

作者：

HENNING, U

SCHWARZ, H

CHEN, R

机构：

[1] Max-Planck-Institut für Biologie

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 97卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90339-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and sensitive radioimmunoassay is described which can detect insoluble membrane proteins in single colonies of Escherichia coli K-12. The method involves transfer of colonies onto filter paper, extraction with organic solvents, exposure to radioiodinated specific immunoglobulin, and autoradiography. Some 30,000 colonies can easily be screened within a week. The method should be applicable for shot-gun-type cloning experiments aiming at genes for insoluble membrane proteins and when selection for a corresponding wild-type allelc is not possible. © 1979.

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页码：153 / 157

页数：5

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