Cloning of a cDNA encoding rat aldehyde dehydrogenase with high activity for retinal oxidation

被引：68
|
作者：
Bhat, PV
Labrecque, J
Boutin, JM
Lacroix, A
Yoshida, A
机构：
[1] UNIV MONTREAL,HOTEL DIEU MONTREAL,DEPT MED,CTR RECH,MOLEC ENDOCRINOL LAB,MONTREAL,PQ H2W 1T8,CANADA
[2] BECKMAN RES INST CITY HOPE,DEPT BIOCHEM GENET,DUARTE,CA 90110
来源：
GENE | 1995年 / 166卷 / 02期
关键词：
recombinant DNA; nucleotide sequence; northern blot; tissue distribution; reverse transcription-polymerase chain reaction;
D O I：
10.1016/0378-1119(96)81752-5
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Retinoic acid (RA), an important regulator of cell differentiation, is biosynthesized from retinol via retinal by a two-step oxidation process, We previously reported the purification and partial amino acid (aa) sequence of a rat kidney aldehyde dehydrogenase (ALDH) isozyme that catalyzed the oxidation of 9-cis and all-trans retinal to corresponding RA with high efficiency [Labrecque et al. Biochem. J. 305 (1995) 681-684]. A rat kidney cDNA library was screened using a 291-bp PCR product generated from total kidney RNA using a pair of oligodeoxyribonucleotide primers matched with the aa sequence. The full-length rat kidney ALDH cDNA contains a 2315-bp (501 aa) open reading frame (ORF), The aa sequence of rat kidney ALDH is 89, 96 and 87% identical to that of the rat cytosolic ALDH, the mouse cytosolic ALDH and human cytosolic ALDH, respectively, Northern blot and RT-PCR-mediated analysis demonstrated that rat kidney ALDH is strongly expressed in kidney, lung, testis, intestine, stomach and trachea, but weakly in the liver.
引用
收藏
页码：303 / 306
页数：4
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