ADSORPTION OF OLIGONUCLEOTIDES ONTO POLYISOHEXYLCYANOACRYLATE NANOPARTICLES PROTECTS THEM AGAINST NUCLEASES AND INCREASES THEIR CELLULAR UPTAKE

被引：112

作者：

CHAVANY, C

SAISONBEHMOARAS, T

LEDOAN, T

PUISIEUX, F

COUVREUR, P

HELENE, C

机构：

[1] MUSEUM NATL HIST NAT, BIOPHYS LAB, CNRS, URA 481, INSERM, U201, F-75231 PARIS 05, FRANCE

[2] MUSEUM NATL HIST NAT, CHIM LAB, F-75005 PARIS, FRANCE

[3] UNIV PARIS SUD, PHYSICCHIM SURFACES & INNOVAT PHARMACOTECH, CNRS, URA 1218, F-92296 CHATENAY MALABRY, FRANCE

来源：

PHARMACEUTICAL RESEARCH | 1994年 / 11卷 / 09期

关键词：

OLIGODEOXYNUCLEOTIDES; NANOPARTICLES; POLYALKYLCYANOACRYLATE; CELLULAR UPTAKE; OLIGONUCLEOTIDE HYDROLYSIS;

D O I：

10.1023/A:1018923301967

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Oligonucleotides can be adsorbed on polyisohexylcyanoacrylate nanoparticles in the presence of hydrophobic quartenary ammonium salts. Oligonucleotides bound to nanoparticles are protected from nuclease attack both in buffer and in cell culture media. Cellular uptake of oligonucleotides is increased when they are adsorbed onto nanoparticles as a result of the capture of nanoparticles by an endocytic/phagocytic pathway. Intracellular stability towards nucleolytic degradation is increased in the presence of nanoparticles. These results show that nanoparticles can be considered as convenient carriers for the protection and delivery of oligonucleotides to cells.

引用

页码：1370 / 1378

页数：9

共 31 条

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