ANALYSIS OF EXCITATION-CONTRACTION-COUPLING COMPONENTS IN CHRONICALLY STIMULATED CANINE SKELETAL-MUSCLE

被引:54
作者
OHLENDIECK, K
BRIGGS, FN
LEE, KF
WECHSLER, AW
CAMPBELL, KP
机构
[1] UNIV IOWA, COLL MED, HOWARD HUGHES MED INST, 400 ECKSTEIN MED RES BLDG, IOWA CITY, IA 52242 USA
[2] UNIV IOWA, COLL MED, DEPT PHYSIOL & BIOPHYS, IOWA CITY, IA 52242 USA
[3] VIRGINIA COMMONWEALTH UNIV, MED COLL VIRGINIA, DEPT PHYSIOL, RICHMOND, VA 23298 USA
[4] VIRGINIA COMMONWEALTH UNIV, MED COLL VIRGINIA, DEPT SURG, RICHMOND, VA 23298 USA
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 202卷 / 03期
关键词
D O I
10.1111/j.1432-1033.1991.tb16428.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The chronic stimulation of predominantly fast-twitch mammalian skeletal muscle causes a transformation to physiological characteristics of slow-twitch skeletal muscle. Here, we report the effects of chronic stimulation on the protein components of the sarcoplasmic reticulum and transverse tubular membranes which are directly involved in excitation-contraction coupling. Comparison of protein composition of microsomal fractions from control and chronically stimulated muscle was performed by immunoblot analysis and also by staining with Coomassie blue or the cationic carbocyanine dye Stains-all. Consistent with previous experiments, a greatly reduced density was observed for the fast-twitch isozyme of Ca2+-ATPase, while the expression of the slow-twitch Ca2+-ATPase was found to be greatly enhanced. Components of the sarcolemma (Na+/K+-ATPase, dystrophin-glycoprotein complex) and the free sarcoplasmic reticulum (Ca2+-binding protein sarcalumenin and a 53-kDa glycoprotein) were not affected by chronic stimulation. The relative abundance of calsequestrin was slightly reduced in transformed skeletal muscle. However, the expression of the ryanodine receptor/Ca2+-release channel from junctional sarcoplasmic reticulum and the transverse tubular dihydropyridine-sensitive Ca2+ channel, as well as two junctional sarcoplasmic reticulum proteins of 90 kDa and 94 kDa, was greatly suppressed in transformed muscle. Thus, the expression of the major protein components of the triad junction involved in excitation-contraction coupling is suppressed, while the expression of other muscle membrane proteins is not affected in chronically stimulated muscle.
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页码:739 / 747
页数:9
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