RECOVERY OF CANOLA-MEAL PROTEINS BY PRECIPITATION

被引：13

作者：

CHEN, M ^{[1
]}

ROHANI, S ^{[1
]}

机构：

[1] UNIV SASKATCHEWAN,DEPT CHEM ENGN,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 40卷 / 01期

关键词：

RAPESEED PROTEIN RECOVERY; ISOELECTRIC PRECIPITATION; HMP; CMC; AMMONIUM SULFATE; NITROGEN YIELD; AGGREGATE SIZE;

D O I：

10.1002/bit.260400110

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recovery of rapeseed proteins from defatted canola meal by precipitation was investigated. The ability of different precipitating agents, such as sodium hexametaphosphate (HMP), carboxymethylcellulose (CMC), ammonium sulphate, and isoelectric precipitation using HCl, were evaluated based on the yield and mean size of protein aggregates. Almost 94% of dissolved protein was precipitated in the presence of 2.7M ammonium sulphate, while the largest mean protein particle size (32-mu-m) was obtained in the presence of HMP at pH 3.3.

引用

页码：63 / 68

页数：6

共 15 条

[1]

Bell D. J., 1983, Advances in Biochemical Engineering/Biotechnology, V26, P1

[2] PROTEINS OF RAPESEED (BRASSICA NAPUS L) SOLUBLE IN SALT SOLUTIONS [J].

BHATTY, RS ;

MCKENZIE, SL ;

FINLAYSON, AJ .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1968, 46 (10) :1191-+

[3] RAPESEED PROTEIN ISOLATES - EFFECT OF PROCESSING ON YIELD AND COMPOSITION OF PROTEIN [J].

BLAICHER, FM ;

ELSTNER, F ;

STEIN, W ;

MUKHERJEE, KD .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1983, 31 (02) :358-362

[4] THE KINETICS OF PROTEIN PRECIPITATION BY DIFFERENT REAGENTS [J].

CHAN, MYY ;

HOARE, M ;

DUNNILL, P .

BIOTECHNOLOGY AND BIOENGINEERING, 1986, 28 (03) :387-393

[5] POLY-ELECTROLYTE PRECIPITATION OF PROTEINS .1. THE EFFECTS OF REACTOR CONDITIONS [J].

FISHER, RR ;

GLATZ, CE .

BIOTECHNOLOGY AND BIOENGINEERING, 1988, 32 (06) :777-785

[6] PREPARATION OF RAPESEED PROTEIN ISOLATES - DISSOLUTION AND PRECIPITATION BEHAVIOR OF RAPESEED PROTEINS [J].

GILLBERG, L ;

TORNELL, B .

JOURNAL OF FOOD SCIENCE, 1976, 41 (05) :1063-1069

[7] PREPARATION OF RAPESEED PROTEIN ISOLATES - PRECIPITATION OF RAPESEED PROTEINS IN PRESENCE OF POLYACIDS [J].

GILLBERG, L ;

TORNELL, B .

JOURNAL OF FOOD SCIENCE, 1976, 41 (05) :1070-1075

[8]

GILLBERG L, 1974, 4 P INT C FOOD SCI T, V5, P142

[9] THE REACTION BETWEEN PHYTATE AND PROTEIN [J].

HILL, R ;

TYLER, C .

JOURNAL OF AGRICULTURAL SCIENCE, 1954, 44 (03) :324-326

[10] REACTOR DESIGN FOR PROTEIN PRECIPITATION AND ITS EFFECT ON CENTRIFUGAL SEPARATION [J].

HOARE, M ;

DUNNILL, P ;

BELL, DJ .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1983, 413 (DEC) :254-269

← 1 2 →