MICRODETERMINATION OF PROTEIN IN HUMAN SERUM AND URINE BY SPECTROFLUOROMETRIC METHOD

A sensitive spectrofluorimetric method of protein determination in human serum and urine was proposed. Intensive fluorescence of terbium(III) (lambda = 545 nm) was observed after excitation of the system (lambda = 328 nm). The method is satisfactory for the determination of protein in the range of 1.1-51.0-mu-g.ml-1 (1.1 x 10(-9)-5.1 x 10(-8) mol.l-1). Protein may be determined in buffer solutions, five days after the sample preparation. A great advantage of this method is an application of microsamples in protein determination in human serum (0.1-mu-l) and human urine (10-mu-l). The effect of other metal ions and organic compounds upon the intensity of the fluorescence emitted by conjugated complex was discussed.