SEQUENCE-ANALYSIS REVEALS HOMOLOGY BETWEEN 2 PROTEINS OF THE FLAGELLAR RADIAL SPOKE

被引:52
作者
CURRY, AM [1 ]
WILLIAMS, BD [1 ]
ROSENBAUM, JL [1 ]
机构
[1] YALE UNIV,DEPT BIOL,NEW HAVEN,CT 06511
关键词
D O I
10.1128/MCB.12.9.3967
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Flagellar radial spokes contribute to the regulation of dynein arm activity and thus the pattern of flagellar bending. We have sequenced the genes for radial spoke protein 4 (RSP4) and RSP6, two of the five proteins that make up the radial spoke head in Chlamydomonas reinhardtii. The two genes, which are tightly linked genetically (B. Huang, G. Piperno, Z. Ramanis, and D. J. L. Luck, J. Cell Biol. 88:80-88, 1981), are separated by only 435 bp. They encode proline-rich polypeptides of 49.8 kDa (RSP4) and 48.8 kDa (RSP6), which are 48% identical to each other but do not resemble any previously sequenced proteins. The transcription start sites of these genes and an additional radial spoke protein gene, that for RSP3, were determined, and patterns of mRNA accumulation during flagellar regeneration were examined for the three radial spoke protein genes. These studies provide the molecular tools for a detailed analysis of radial spoke head function and assembly and for a determination of the mechanism by which the genes required to build a complex organelle are regulated.
引用
收藏
页码:3967 / 3977
页数:11
相关论文
共 57 条
[1]   CELL-WALL REGENERATION IN CHLAMYDOMONAS - ACCUMULATION OF MESSENGER-RNAS ENCODING CELL-WALL HYDROXYPROLINE-RICH GLYCOPROTEINS [J].
ADAIR, WS ;
APT, KE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) :7355-7359
[2]  
Ausubel FM, 1987, CURRENT PROTOCOLS MO
[4]   PROTEIN-SYNTHESIS IS REQUIRED FOR RAPID DEGRADATION OF TUBULIN MESSENGER-RNA AND OTHER DEFLAGELLATION-INDUCED RNAS IN CHLAMYDOMONAS-REINHARDI [J].
BAKER, EJ ;
KELLER, LR ;
SCHLOSS, JA ;
ROSENBAUM, JL .
MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (01) :54-61
[5]   ANALYSIS OF THE MOVEMENT OF CHLAMYDOMONAS FLAGELLA - THE FUNCTION OF THE RADIAL-SPOKE SYSTEM IS REVEALED BY COMPARISON OF WILD-TYPE AND MUTANT FLAGELLA [J].
BROKAW, CJ ;
LUCK, DJL ;
HUANG, B .
JOURNAL OF CELL BIOLOGY, 1982, 92 (03) :722-732
[6]   REPEATED CONSENSUS SEQUENCE AND PSEUDOPROMOTERS IN THE 4 COORDINATELY REGULATED TUBULIN GENES OF CHLAMYDOMONAS-REINHARDI [J].
BRUNKE, KJ ;
ANTHONY, JG ;
STERNBERG, EJ ;
WEEKS, DP .
MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (06) :1115-1124
[7]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[8]   RESCUE OF A PARALYZED-FLAGELLA MUTANT OF CHLAMYDOMONAS BY TRANSFORMATION [J].
DIENER, DR ;
CURRY, AM ;
JOHNSON, KA ;
WILLIAMS, BD ;
LEFEBVRE, PA ;
KINDLE, KL ;
ROSENBAUM, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) :5739-5743
[9]   A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].
FEINBERG, AP ;
VOGELSTEIN, B .
ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13
[10]   ISOLATION AND CHARACTERIZATION OF THE NITRATE REDUCTASE STRUCTURAL GENE OF CHLAMYDOMONAS-REINHARDTII [J].
FERNANDEZ, E ;
SCHNELL, R ;
RANUM, LPW ;
HUSSEY, SC ;
SILFLOW, CD ;
LEFEBVRE, PA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (17) :6449-6453