DETERMINATION OF BARLEY PROLAMINS IN BEER AND BREWING MATERIALS

被引：2

作者：

Hulin, Petr ^{[1
]}

Dostalek, Pavel ^{[1
]}

Hochel, Igor ^{[2
]}

机构：

[1] Inst Chem Technol Prague, Dept Fermentat Chem & Bioengn, Tech 5, Prague 16628 6, Czech Republic

[2] Inst Chem Technol Prague, Dept Biochem & Microbiol, Tech 5, Prague 16628 6, Czech Republic

来源：

KVASNY PRUMYSL | 2007年 / 53卷 / 09期

关键词：

prolamins; barley; beer;

D O I：

10.18832/kp2007015

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The work deals with the possibilities of immunochemical detection of hordein fractions of barley proteins and possible cleavage derivates, or more precisely by characterization of monoclonal antibodies prepared for the purpose of a rapid, specific and sensitive determination of hordein proteins in the food industry, specifically in beer. A method of indirect competitive ELISA for barley prolamins has been developed and working concentrations of all immunoagents have been determined. Further to this, cross interactions of particular monoclonal antibodies with the proteins of the other cereals have been tested. The detection limit of the method was 2 ppm with the linear range of the calibration curve ranging from 1 to 100 ppm.

引用

页码：273 / 276

页数：4

共 6 条

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