DEPHOSPHORYLATION OF HUMAN P34(CDC2) KINASE ON BOTH THR-14 AND TYR-15 BY HUMAN CDC25B PHOSPHATASE

被引：88
作者：
HONDA, R
OHBA, Y
NAGATA, A
OKAYAMA, H
YASUDA, H
机构：
[1] KANAZAWA UNIV, FAC PHARMACEUT SCI, DIV BIOL, KANAZAWA, ISHIKAWA 920, JAPAN
[2] UNIV TOKYO, FAC MED, DEPT BIOCHEM, TOKYO 113, JAPAN
来源：
FEBS LETTERS | 1993年 / 318卷 / 03期
关键词：
CDC2; KINASE; CDC25; PROTEIN PHOSPHORYLATION; PHOSPHATASE;
D O I：
10.1016/0014-5793(93)80540-B
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In mammalian cells, p34cdc2 kinase undergoes phosphorylation at threonine- 1 4, tyrosine-15 and threonine- 1 61 in the S and G2 phases of the cell cycle. At the onset of mitosis, the kinase becomes dephosphorylated at threonine-14 and tyrosine-15, resulting in activation. Cdc25 phosphatase has been shown to dephosphorylate tyrosine-15 in vitro, but whether it also does at threonine-14 remains unclear. In this study, we have found that human cdc25B phosphatase dephosphorylates both threonine-14 and tyrosine-15 but not threonine-161.
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页码：331 / 334
页数：4
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