SELECTIVE COUPLING OF THE HUMAN ANAPHYLATOXIN C5A RECEPTOR AND ALPHA-16 IN HUMAN KIDNEY 293 CELLS

被引：58
作者：
BUHL, AM
EISFELDER, BJ
WORTHEN, GS
JOHNSON, GL
RUSSELL, M
机构：
[1] NATL JEWISH CTR IMMUNOL & RESP MED,DIV BASIC SCI,1400 JACKSON ST,DENVER,CO 80206
[2] NATL JEWISH CTR IMMUNOL & RESP MED,DEPT MED,DENVER,CO 80206
[3] AARHUS UNIV,INST CHEM,DEPT BIOSTRUCT CHEM,DK-8000 AARHUS,DENMARK
[4] UNIV COLORADO,SCH MED,DEPT PHARMACOL,DENVER,CO 80262
[5] UNIV COLORADO,SCH MED,DEPT MED,DENVER,CO 80262
来源：
FEBS LETTERS | 1993年 / 323卷 / 1-2期
关键词：
C5A RECEPTOR; G-ALPHA-16; HUMAN KIDNEY 293 CELL;
D O I：
10.1016/0014-5793(93)81464-B
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The peptide C5a which is generated during the complement cascade is an important chemotactic factor involved in the inflammatory response. The C5a receptor (C5aR) primary sequence suggests that it has a serpentine structure of seven transmembrane domains which is typical of classical G-protein-coupled receptors. To investigate the signal transduction mechanism of C5a we transiently expressed the C5aR in combination with different G-protein alpha subunits in human kidney 293 cells and measured the PLC activity induced upon C5a stimulation. Cotransfection of C5aR and alpha16 stimulated PLC while cotransfection of C5aR with either alpha(q) or alpha(i2) did not.
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页码：132 / 134
页数：3
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