X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN LEUPEPTIN COMPLEX

被引：121

作者：

SCHRODER, E ^{[1
]}

PHILLIPS, C ^{[1
]}

GARMAN, E ^{[1
]}

HARLOS, K ^{[1
]}

CRAWFORD, C ^{[1
]}

机构：

[1] UNIV OXFORD,MOLEC BIOPHYS LAB,REX RICHARDS BLDG,S PARKS RD,OXFORD OX1 3QU,ENGLAND

来源：

FEBS LETTERS | 1993年 / 315卷 / 01期

关键词：

LEUPEPTIN; PAPAIN; ENZYME-INHIBITOR COMPLEX; OXYANION HOLE; HEMITHIOACETAL; X-RAY CRYSTALLOGRAPHY;

D O I：

10.1016/0014-5793(93)81128-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The three-dimensional structure of the papain-leupeptin complex has been determined by X-ray crystallography to a resolution of 2.1 angstrom (overall R-factor = 19.8%). The structure indicates that: (i) leupeptin contacts the S subsites of the papain active site and not the S' subsites; (ii) the 'carbonyl' carbon atom of the inhibitor is covalently bound by the Cys-25 sulphur atom of papain and is tetrahedrally coordinated; (iii) the 'carbonyl' oxygen atom of the inhibitor faces the oxyanion hole and makes hydrogen bond contacts with Gln-19 and Cys-25.

引用

页码：38 / 42

页数：5

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