SPONTANEOUS BACTERICIDAL AND COMPLEMENT ACTIVITIES IN SERUM OF RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) GENETICALLY SELECTED FOR RESISTANCE OR SUSCEPTIBILITY TO FURUNCULOSIS

被引：38
作者：
HOLLEBECQ, MG [1 ]
FAIVRE, B [1 ]
BOURMAUD, C [1 ]
MICHEL, C [1 ]
机构：
[1] INRA,CTR RECH JOUY EN JOSAS,UNITE VIROL & IMMUNOL MOLEC,F-78352 JOUY EN JOSAS,FRANCE
来源：
FISH & SHELLFISH IMMUNOLOGY | 1995年 / 5卷 / 06期
关键词：
ONCORHYNCHUS MYKISS; BACTERICIDAL SERUM ACTIVITY; AEROMONAS SALMONICIDA; FURUNCULOSIS; ALTERNATIVE COMPLEMENT PATHWAY; DISEASE RESISTANCE;
D O I：
10.1006/fsim.1995.0040
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Half-sib families of rainbow trout (Oncorhynchus mykiss) selected for heritable susceptibility or resistance to furunculosis and belonging to 3 successive generations were reared in a fish farm and blood samples were periodically collected. Pooled and individual sera were screened for the effectiveness of two kinds of activity generally assigned to the alternative complement pathway: bactericidal activity was assessed against suspensions of an avirulent form of Aeromonas salmonicida and spontaneous haemolytic activity (SH50) was titrated using rabbit erythrocytes in the presence of EGTA and Mg2+. Significant differences in serum bactericidal properties generally occurred between susceptible and resistant families of trout, although transient variations could occur. Seasonal fluctuations, irrespective of the age of the fish, were also observed. However, whereas correlation between the bactericidal activity or alternative complement pathway and the fish resistance could be observed, it was more difficult to demonstrate between the bactericidal activity and the alternative complement pathway. It is concluded that the alternative complement activity, although significant in natural defences, accounts only partly for furunculosis resistance and that bactericidal activity of serum is a more reliable marker of this resistance in rainbow trout. (C) 1995 Academic Press Limited
引用
收藏
页码：407 / 426
页数：20
相关论文
共 49 条
  • [1] Anderson D.P., Jeney G., Immunostimulants added to injected Aeromonas salmonicida bacterin enhance the defense mechanisms and protection in rainbow trout (Oncorhynchus mykiss), Veterinary Immunology and Immuno-Pathology, 34, pp. 379-389, (1992)
  • [2] Bower S.M., Evelyn T.P.T., Acquired and innate resistance to the haemoflagellate Cryptobia salmositica in sockeye salmon (Oncorhynchus nerka), Developmental and Comparative Immunology, 12, pp. 749-760, (1988)
  • [3] Chevassus B., Dorson M., Genetics of resistance to disease in fishes, Aquaculture, 85, pp. 83-107, (1990)
  • [4] Cipriano R., Resistance of salmonids to Aeromonas salmonicida: Relation between agglutinins and neutralizing activities, Transactions of the American Fisheries Society, 112, pp. 95-99, (1983)
  • [5] Cipriano R., Heartwell C.M., Susceptibility of salmonids to furunculosis: Differences between serum and mucus responses against, Aeromonas Salmonicida. Transactions of the American Fisheries Society, 115, pp. 83-88, (1986)
  • [6] Dorson M., Torchy C., Michel C., Rainbow trout complement fixation used for titration of antibodies against several pathogens, Annales Recherche Veterinaire, 10, pp. 529-534, (1979)
  • [7] Ellis A.E., Hastings T.S., Munro A.L.S., The role of Aeromonas salmonicida extracellular products in the pathology of furunculosis, Journal of Fish Diseases, 4, pp. 41-51, (1981)
  • [8] Fevolden S.E., Refstie T., Roed K.H., Disease resistance in rainbow trout (Oncorhynchus mykiss) selected for high or low responses to stress, Aquaculture, 104, pp. 19-29, (1992)
  • [9] Fevolden S.E., Nordmo R., Refstie T., Roed K.H., Disease resistance in Atlantic salmon (Salmo salai) selected for high or low responses to stress, Aquaculture, 109, pp. 215-224, (1993)
  • [10] Fevolden S.E., Roed K.H., Gjerde B., Genetic components of post-stress cortisol and lysozyme activity in Atlantic salmon
12345