LONG-LASTING EFFECTS OF AUDIOGENIC-SEIZURES ON BIOGENIC-AMINES IN RB MICE

The existence of long-lasting (15-18 h) alterations of biogenic amines levels following a single or repeated acoustic stimulations in audiogenic seizure-prone Rbl and Rb2 mice and seizure-resistant Rb3 mice were investigated. The levels of 5-HT, DA, NE, and of some of their catabolites: 5-HIAA, DOPAC, were determined, as well as changes in synthesis and utilization indices of 5-HT and DA, in 14 brain areas. Increased NE Levels were observed after a single seizure in Rb2 mice, in the frontal cortex, hypothalamus, raphe and after repeated seizures in Rbl in frontal cortex, raphe, striatum and in Rb2 in hypothalamus, inferior colliculus. Only rare alterations of 5-HT neurotransmission were recorded after a single or repeated seizures, in the raphe and superior colliculus. DA neurotransmission is more affected after repeated seizures, namely in frontal cortex, striatum, hypothalamus, inferior colliculus, raphe, olfactory tubercles, amygdala, and thalamus than after a single one (frontal cortex, amygdala, thalamus, raphe). In Rb3 mice, resistant to sonic stimulations, biogenic amines alterations were rare. Taken all together, a single seizure induced scarce long-lasting alterations in biogenic amines levels. They were more frequent after repeated seizure.