ISOLATION OF A NEW GENE (SWA2) ENCODING AN ALPHA-AMYLASE FROM SCHWANNIOMYCES-OCCIDENTALIS AND ITS EXPRESSION IN SACCHAROMYCES-CEREVISIAE

被引：18

作者：

ABARCA, D ^{[1
]}

FERNANDEZLOBATO, M ^{[1
]}

DELPOZO, L ^{[1
]}

JIMENEZ, A ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,CSIC,CTR BIOL MOLEC,CANTO BLANCO,E-28049 MADRID,SPAIN

来源：

FEBS LETTERS | 1991年 / 279卷 / 01期

关键词：

ALPHA-AMYLASE; GENE CLONING; STARCH UTILIZATION; SCHWANNIOMYCES-OCCIDENTALIS; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1016/0014-5793(91)80245-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new gene (SW A2) encoding a secretory alpha-amylase activity from Schwanniomyces occidentalis has been cloned from this yeast and then expressed in Saccharomyces cerevisiae. Both Sw. occidentalis and a transformant of S. cerevisiae incorporating SW A2 contain a transcript of 2.1 kb which hybridizes to DNA carrying the SW A2 gene. This indicates that the transcript is a product of the SW A2 gene. Transcription of the SW A2 gene seems to be regulated in both Sw. occidentalis and S. cerevisiae. Furthermore, the secretion of alpha-amylase activity is drastically repressed by glucose in both Sw. occidentalis and a transformant of S. cerevisiae containing SW A2.

引用

页码：41 / 44

页数：4

共 21 条

[1] ISOLATION AND EXPRESSION IN SACCHAROMYCES-CEREVISIAE OF A GENE ENCODING AN ALPHA-AMYLASE FROM SCHWANNIOMYCES-CASTELLII [J].

ABARCA, D ;

FERNANDEZLOBATO, M ;

CLAROS, MG ;

JIMENEZ, A .

FEBS LETTERS, 1989, 255 (02) :455-459

[2] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].

AVIV, H ;

LEDER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (06) :1408-&

[3]

BROACH JR, 1979, GENE, V8, P121, DOI 10.1016/0378-1119(79)90012-X

[4] TRANSFORMATION OF YEAST SPHEROPLASTS WITHOUT CELL-FUSION [J].

BURGERS, PMJ ;

PERCIVAL, KJ .

ANALYTICAL BIOCHEMISTRY, 1987, 163 (02) :391-397

[5]

CONSTANZE C, 1987, METHOD ENZYMOL, V152, P582

[6] REGULATED OVERPRODUCTION OF ALPHA-AMYLASE BY TRANSFORMATION OF THE AMYLOLYTIC YEAST SCHWANNIOMYCES-OCCIDENTALIS [J].

DOHMEN, RJ ;

STRASSER, AWM ;

ZITOMER, RS ;

HOLLENBERG, CP .

CURRENT GENETICS, 1989, 15 (05) :319-325

[7]

EMONI M, 1988, AGR BIOCH CHEM, V52, P399

[8] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[9] MOLECULAR-CLONING AND CHARACTERIZATION OF THE STREPTOMYCES-HYGROSCOPICUS ALPHA-AMYLASE GENE [J].

HOSHIKO, S ;

MAKABE, O ;

NOJIRI, C ;

KATSUMATA, K ;

SATOH, E ;

NAGAOKA, K .

JOURNAL OF BACTERIOLOGY, 1987, 169 (03) :1029-1036

[10]

HOSHIKO S, 1989, J BACTERIOL, V255, P455

← 1 2 3 →