CLONING AND FUNCTIONAL-CHARACTERIZATION OF THE HUMAN VASOACTIVE-INTESTINAL-PEPTIDE (VIP)-2 RECEPTOR

被引：91
作者：
ADAMOU, JE
AIYAR, N
VANHORN, S
ELSHOURBAGY, NA
机构：
[1] SMITHKLINE BEECHAM PHARMACEUT,DEPT MOLEC GENET,KING OF PRUSSIA,PA 19406
[2] SMITHKLINE BEECHAM PHARMACEUT,DEPT PHARMACOL,KING OF PRUSSIA,PA 19406
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 209卷 / 02期
关键词：
D O I：
10.1006/bbrc.1995.1515
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A full-length clone encoding the human VIP-2 receptor was isolated from a human placenta cDNA Library. The 1317-bp cDNA insert encodes a protein of 438 amino acids which is a member of the seven transmembrane domain G-protein-coupled receptor superfamily. Expression of the human VIP-2 receptor in COS-7 cells confered high affinity binding to [I-125] VIP (IC50 = 0.93 nM), which was displaced by unlabeled PACAP-38 (IC50 = 6.2 nM). VIP and PACAP-38 were equipotent in stimulating accumulation of cAMP in COS-7 cells transfected with the human VIP-2 receptor. Northern blot analysis revealed two VIP-2 receptor mRNAs of 4.6 kb and 2.3 kb in size which were expressed predominantly in the human skeletal muscle and to a lesser extent in the human brain, heart, pancreas and placenta. (C) 1995. Academic Press, Inc.
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页码：385 / 392
页数：8
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