FORMATION OF F-2-ISOPROSTANES IN OXIDIZED LOW-DENSITY-LIPOPROTEIN - INHIBITORY EFFECT OF HYDROXYTYROSOL

被引：75

作者：

SALAMI, M ^{[1
]}

GALLI, C ^{[1
]}

DEANGELIS, L ^{[1
]}

VISIOLI, F ^{[1
]}

机构：

[1] INST PHARMACOL SCI,I-20133 MILAN,ITALY

来源：

PHARMACOLOGICAL RESEARCH | 1995年 / 31卷 / 05期

关键词：

OXIDIZED LDL; ISOPROSTANES; OLIVE OIL; MEDITERRANEAN DIET; HYDROXYTYROSOL;

D O I：

10.1016/1043-6618(95)80032-8

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Oxidatively-modified low-density lipoproteins (LDL) contribute to the onset of the atherosclerotic disease. A recently discovered marker of lipid peroxidation is a series of prostaglandin F-2-like compounds, the prostaglandin isomers isoprostanes, that are generated from arachidonic acid through cyclooxygenase-independent pathways following free radical injury and are endowed with potent biological activities. The incidence of cardiovascular disease in the Mediterranean area is low, possibly because of the type of fat (mainly olive oil) and other components (e.g. fruits and vegetables) of the diet. Natural antioxidants abound in this kind of diet and may also contribute to the observed protection from coronary heart disease (CHD) by retarding the formation of the atherosclerotic plaque. Olive oil, the major dietary fat in the Mediterranean countries, is rich in phenols with antioxidant properties. We thus investigated the formation of isoprostanes during in vitro LDL oxidation and tested the effect of an olive-oil-extracted phenol (i.e. hydroxytyrosol). Our data show that production of isoprostanes and other markers of lipid peroxidation occurs during LDL oxidation and is inhibited by hydroxytyrosol.

引用

页码：275 / 279

页数：5

共 20 条

[1]

Steinberg, Parthasarathy, Carew, Khoo, Witzum, Beyond cholesterol. Modifications of lowdensity lipoprotein that increase its atherogenicity, N Engl J Med, 320, pp. 915-924, (1989)

[2]

Morrow, Hill, Burk, Nammour, Badr, Roberts, A series of prostaglandin F2-like compounds are produced in vivo in humans by a noncyclooxygenase, free radical-catalyzed mechanism, Proc Natl Acad Sci USA, 87, pp. 9383-9387, (1990)

[3]

Morrow, Awad, Boss, Blair, Roberts, Non-cyclooxygenase-derived prostanoids F2-isopro-stanes) are formed in situ on phospholipids, Proc Natl Acad Sci USA, 89, pp. 10721-10725, (1992)

[4]

Banerjee, Kang, Morrow, Roberts, Newman, Effects of a novel prostaglandin, 8-epiPGF2α, in rabbit lung in situ, Am J Physiol, 263, pp. H660-H663, (1992)

[5]

Morrow, Minton, Roberts, The F2-isoprostane, 8-epi-PGF2α, a potent agonist of the vascular thromboxane/endoperoxide receptor, is a platelet thromboxane/endoperoxide receptor antagonist, Prostaglandins, 44, pp. 155-163, (1992)

[6]

Lynch, Morrow, Roberts, Frei, Formation of non-cyclooxygenase-derived prostandoids F2isoprostanes) in plasma and low density lipoprotein exposed to oxidative stress in vitro, J Clin Invest, 93, pp. 998-1004, (1994)

[7]

Morrow, Minton, Badr, Minton, Jackson Roberts, Evidence that F2-isoprostane, 8-epiPGF2α, is formed in vivo, Biochem Biophys Acta, 1210, pp. 244-248, (1994)

[8]

Montedoro, Servili, Baldioli, Miniati, Simple and hydrolyzable phenolic compounds in virgin olive oil. 1. Their extraction, separation, and quantitative and semiquantitative evaluation by HPLC, J Agric Food Chem, 40, pp. 1571-1576, (1992)

[9]

Tiscornia, Forina, Evangelisti, Composizione chimica dell' olio di oliva e sue variazioni indotte dal processo di rettificazione, Riv Ital Sistanze Grasse, 54, pp. 519-556, (1982)

[10]

Hertog, Feskens, Katan, Kromhout, Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study, Lancet, 342, pp. 1007-1011, (1993)

← 1 2 →