FLOW CYTOMETRIC ANALYSIS OF LYMPHOCYTE-T SUBSETS IN CATS

被引：67

作者：

DEAN, GA ^{[1
]}

QUACKENBUSH, SL ^{[1
]}

ACKLEY, CD ^{[1
]}

COOPER, MD ^{[1
]}

HOOVER, EA ^{[1
]}

机构：

[1] UNIV ALABAMA, INST TUMOR, BIRMINGHAM, AL 35294 USA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1991年 / 28卷 / 3-4期

关键词：

NUMBER; CELLS;

D O I：

10.1016/0165-2427(91)90124-U

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We report a rapid, reliable method for the immunophenotype analysis of feline lymphocytes. Fluorescein isothiocyanate (FITC) conjugated to murine monoclonal antibodies f43, Fel 7 and fCD8 was used to identify phenotypes corresponding to feline T-cells, CD4+ T cells and CD8+ T cells. For isolation of white blood cells, whole blood lysis was faster, less variable and required much less sample than density gradient separation. To identify feline CD4 + and CD8 + cells simultaneously, directly conjugated FITC-fCD8 and phycoerythrin (PE) fCD4 (Fel 7) were used in two-color analysis. The two T cell sub-populations were non-overlapping. Dual-label and single-label values were not significantly different. Mean lymphocyte subset percentages in conventional and specific-pathogen-free (SPF) cats did not differ significantly. These values were: pan T lymphocytes (f43), 54.8%, CD4 + cells (Fel 7), 33.9%, and CD8+ cells (fCD8), 19.1%. Mean CD4/CD8 ratio was 1.9 in normal cats; the range was 1.2-2.6

引用

页码：327 / 335

页数：9

共 10 条

[1] IDENTIFICATION OF A CD4 HOMOLOG IN THE CAT [J].