SIMULTANEOUS LOCALIZATION OF COSMIDS AND CHROMOSOME R-BANDING BY FLUORESCENCE MICROSCOPY - APPLICATION TO REGIONAL MAPPING OF HUMAN CHROMOSOME-11

被引：147
作者：
CHERIF, D
JULIER, C
DELATTRE, O
DERRE, J
LATHROP, GM
BERGER, R
机构：
[1] CNRS,STRUCT INTERVENT 15954,F-75005 PARIS,FRANCE
[2] CTR ETUD POLYMORPHISME HUMAIN,PARIS,FRANCE
[3] INST CURIE,CNRS,URA 620,F-75231 PARIS 05,FRANCE
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 17期
关键词：
biotin labeling; in situ hybridization;
D O I：
10.1073/pnas.87.17.6639
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A technique for nonradioactive in situ hybridization on human metaphase chromosomes has been developed to localize human cosmid clones. The simple procedure using two fluorescent dyes (fluorescein and propidium iodide) allows the simultaneous identification of chromosomal R-bands and hybridization signal in a single screening of the slides. This technique has been used for rapid correlation of the genetic and physical map of chromosome 11q13-qter in the region of genes responsible for ataxia-telangiectasia and tuberous sclerosis.
引用
收藏
页码：6639 / 6643
页数：5
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