PROKARYOTIC 20-BETA-HYDROXYSTEROID DEHYDROGENASE IS AN ENZYME OF THE SHORT-CHAIN, NONMETALLOENZYME ALCOHOL-DEHYDROGENASE TYPE

被引：77

作者：

MAREKOV, L ^{[1
]}

KROOK, M ^{[1
]}

JORNVALL, H ^{[1
]}

机构：

[1] KAROLINSKA INST,DEPT CHEM 1,S-10401 STOCKHOLM 60,SWEDEN

来源：

FEBS LETTERS | 1990年 / 266卷 / 1-2期

关键词：

Alcohol dehydrogenase; Amino acid sequence; Homology; Protein family; Steroid dehydrogenase; Tyrosine residue;

D O I：

10.1016/0014-5793(90)81504-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The primary structure of 20β-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was determined after FPLC purification of a commercial preparation. Peptides obtained from different proteolytic cleavages were purified by reverse phase HPLC. The 255-residue structure deduced was found to be distantly homologous to those of Drosophila alcohol dehydrogenase and several other dehydrogenases, establishing that prokaryotic 20β-hydroxysteroid dehydrogenase as a member of the 'short-chain alcohol dehydrogenase family'. With the enzymes characterized, the identity is greatest (31-34%) towards 4 other prokaryotic dehydrogenases, but the family also includes mammalian steroid and prostaglandin dehydrogenases. These enzymes are low in Cys and have a strictly conserved Tyr residue that appears to be important. © 1990.

引用

页码：51 / 54

页数：4

共 21 条

[1] HUMAN PLACENTAL 17-BETA-HYDROXYSTEROID DEHYDROGENASE IS HOMOLOGOUS TO NODG PROTEIN OF RHIZOBIUM-MELILOTI [J].

BAKER, ME .

MOLECULAR ENDOCRINOLOGY, 1989, 3 (05) :881-884

[2] SITE-DIRECTED MUTAGENESIS OF GLYCINE-14 AND 2 CRITICAL CYSTEINYL RESIDUES IN DROSOPHILA ALCOHOL-DEHYDROGENASE [J].

CHEN, Z ;

LU, L ;

SHIRLEY, M ;

LEE, WR ;

CHANG, SH .

BIOCHEMISTRY, 1990, 29 (05) :1112-1118

[3] NUCLEOTIDE-SEQUENCE AND REGULATION OF A GENE INVOLVED IN BILE-ACID 7-DEHYDROXYLATION BY EUBACTERIUM SP STRAIN VPI-12708 [J].

COLEMAN, JP ;

WHITE, WB ;

LIJEWSKI, M ;

HYLEMON, PB .

JOURNAL OF BACTERIOLOGY, 1988, 170 (05) :2070-2077

[4] RIBITOL DEHYDROGENASE OF KLEBSIELLA-AEROGENES - SEQUENCE AND PROPERTIES OF WILD-TYPE AND MUTANT STRAINS [J].

DOTHIE, JM ;

GIGLIO, JR ;

MOORE, CB ;

TAYLOR, SS ;

HARTLEY, BS .

BIOCHEMICAL JOURNAL, 1985, 230 (03) :569-578

[5] COMPARISON OF 3-ALPHA-HYDROXYSTEROID AND 20-BETA-HYDROXYSTEROID DEHYDROGENASE-ACTIVITIES OF CORTISONE REDUCTASE OF STREPTOMYCES-HYDROGENANS [J].

EDWARDS, CAF ;

ORR, JC .

BIOCHEMISTRY, 1978, 17 (21) :4370-4376

[6] NUCLEOTIDE-SEQUENCE, TRANSCRIPTION AND DEDUCED FUNCTION OF A GENE INVOLVED IN POLYKETIDE ANTIBIOTIC-SYNTHESIS IN STREPTOMYCES-COELICOLOR [J].

HALLAM, SE ;

MALPARTIDA, F ;

HOPWOOD, DA .

GENE, 1988, 74 (02) :305-320

[7] COMPLETE AMINO-ACID-SEQUENCE OF GLUCOSE-DEHYDROGENASE FROM BACILLUS-MEGATERIUM [J].

JANY, KD ;

ULMER, W ;

FROSCHLE, M ;

PFLEIDERER, G .

FEBS LETTERS, 1984, 165 (01) :6-10

[8] SORBITOL DEHYDROGENASE - THE PRIMARY STRUCTURE OF THE SHEEP-LIVER ENZYME [J].

JEFFERY, J ;

CEDERLUND, E ;

JORNVALL, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 140 (01) :7-16

[9] ALCOHOL DEHYDROGENASES AND ALDEHYDE DEHYDROGENASES [J].

JORNVALL, H ;

HOOG, JO ;

VONBAHRLINDSTROM, H ;

JOHANSSON, J ;

KAISER, R ;

PERSSON, B .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1988, 16 (03) :223-223

[10] EXTENDED SUPERFAMILY OF SHORT ALCOHOL-POLYOL-SUGAR DEHYDROGENASES - STRUCTURAL SIMILARITIES BETWEEN GLUCOSE AND RIBITOL DEHYDROGENASES [J].

JORNVALL, H ;

BAHRLINDSTROM, HV ;

JANY, KD ;

ULMER, W ;

FROSCHLE, M .

FEBS LETTERS, 1984, 165 (02) :190-196

← 1 2 3 →