METABOLIC INTERMEDIATES IN LIVER OF RATS GIVEN LARGE AMOUNTS OF FRUCTOSE OR DIHYDROXYACCTONE

The livers of rats given large injections of fructose or dihydroxyacetone were analyzed for glucose, glycogen, and 14 related metabolites. Dihydroxyacetone produced a large increase in glycogen and a modest increase in glucose and glucose-6-P. Fructose produced no increase in glycogen but a large increase in glucose and glucose-6-P. Changes in intermediates suggest in both cases diversion of part of the injected material into the Embden-Meyerhof pathway. Fructose caused a profound drop in ATP, UTP and UDPG. The fall in ATP is attributed to sequestration of phosphate into fructose-1-P, which increased to 18 mmole/kg. The decrease in ATP is believed to lead in turn to the fall in UTP and UDPG and the failure to deposit glycogen. © 1969.