ANTIPARALLEL SIDE-BY-SIDE HETERODIMER FOR SEQUENCE-SPECIFIC RECOGNITION IN THE MINOR GROOVE OF DNA BY A DISTAMYCIN 1-METHYLIMIDAZOLE-2-CARBOXAMIDE-NETROPSIN PAIR

被引：105

作者：

MRKSICH, M ^{[1
]}

DERVAN, PB ^{[1
]}

机构：

[1] CALTECH, ARNOLD & MABEL BECKMAN LABS CHEM SYNTH, PASADENA, CA 91125 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1993年 / 115卷 / 07期

关键词：

D O I：

10.1021/ja00060a004

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The two peptides distamycin A (D)and 1-methylimidazole-2-carboxamide-netropsin (2-ImN) simultaneously bind the five-base-pair sequence 5'-TGTTA-3'. Footprinting experiments indicate that both molecules are necessary for binding to this sequence while affinity cleaving data define the orientations with which each peptide binds to this sequence. The footprinting and affinity cleaving data are consistent with a model wherein the two peptides bind as a side-by-side antiparallel heterodimer in the minor groove of double-helical DNA.

引用

页码：2572 / 2576

页数：5

共 40 条

[11] H-1-NMR STUDIES ON THE INTERACTION BETWEEN DISTAMYCIN-A AND A SYMMETRICAL DNA DODECAMER [J].

KLEVIT, RE ;

WEMMER, DE ;

REID, BR .

BIOCHEMISTRY, 1986, 25 (11) :3296-3303

[12] BINDING OF AN ANTITUMOR DRUG TO DNA NETROPSIN AND C-G-C-G-A-A-T-T-BRC-G-C-G [J].

KOPKA, ML ;

YOON, C ;

GOODSELL, D ;

PJURA, P ;

DICKERSON, RE .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 183 (04) :553-563

[13] THE MOLECULAR-ORIGIN OF DNA DRUG SPECIFICITY IN NETROPSIN AND DISTAMYCIN [J].

KOPKA, ML ;

YOON, C ;

GOODSELL, D ;

PJURA, P ;

DICKERSON, RE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (05) :1376-1380

[14] QUANTITATIVE ESTIMATION OF THE CONTRIBUTION OF PYRROLCARBOXAMIDE GROUPS OF THE ANTIBIOTIC DISTAMYCIN-A INTO SPECIFICITY OF ITS BINDING TO DNA AT PAIRS [J].

KRYLOV, AS ;

GROKHOVSKY, SL ;

ZASEDATELEV, AS ;

ZHUZE, AL ;

GURSKY, GV ;

GOTTIKH, BP .

NUCLEIC ACIDS RESEARCH, 1979, 6 (01) :289-304

[15] SEQUENCE SPECIFICITY OF ACTINOMYCIN-D AND NETROPSIN BINDING TO PBR322 DNA ANALYZED BY PROTECTION FROM DNASE-I [J].

LANE, MJ ;

DABROWIAK, JC ;

VOURNAKIS, JN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (11) :3260-3264

[16] STRUCTURAL AND DYNAMIC ASPECTS OF BINDING OF A PROTOTYPE LEXITROPSIN TO THE DECADEOXYRIBONUCLEOTIDE D(CGCAATTGCG)2 DEDUCED FROM HIGH-RESOLUTION H-1-NMR STUDIES [J].

LEE, M ;

CHANG, DK ;

HARTLEY, JA ;

PON, RT ;

KROWICKI, K ;

LOWN, JW .

BIOCHEMISTRY, 1988, 27 (01) :445-455

[17] MOLECULAR RECOGNITION BETWEEN OLIGOPEPTIDES AND NUCLEIC-ACIDS - NOVEL IMIDAZOLE-CONTAINING OLIGOPEPTIDES RELATED TO NETROPSIN THAT EXHIBIT ALTERED DNA-SEQUENCE SPECIFICITY [J].

LOWN, JW ;

KROWICKI, K ;

BHAT, UG ;

SKOROBOGATY, A ;

WARD, B ;

DABROWIAK, JC .

BIOCHEMISTRY, 1986, 25 (23) :7408-7416

[18] ORIGINS OF NETROPSIN BINDING-AFFINITY AND SPECIFICITY - CORRELATIONS OF THERMODYNAMIC AND STRUCTURAL DATA [J].

MARKY, LA ;

BRESLAUER, KJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (13) :4359-4363

[19]

Maxam A M, 1980, Methods Enzymol, V65, P499

[20] ANTIPARALLEL SIDE-BY-SIDE DIMERIC MOTIF FOR SEQUENCE-SPECIFIC RECOGNITION IN THE MINOR GROOVE OF DNA BY THE DESIGNED PEPTIDE 1-METHYLIMIDAZOLE-2-CARBOXAMIDE NETROPSIN [J].

MRKSICH, M ;

WADE, WS ;

DWYER, TJ ;

GEIERSTANGER, BH ;

WEMMER, DE ;

DERVAN, PB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) :7586-7590

← 1 2 3 4 →