ANTIPARALLEL SIDE-BY-SIDE HETERODIMER FOR SEQUENCE-SPECIFIC RECOGNITION IN THE MINOR GROOVE OF DNA BY A DISTAMYCIN 1-METHYLIMIDAZOLE-2-CARBOXAMIDE-NETROPSIN PAIR

被引：105

作者：

MRKSICH, M ^{[1
]}

DERVAN, PB ^{[1
]}

机构：

[1] CALTECH, ARNOLD & MABEL BECKMAN LABS CHEM SYNTH, PASADENA, CA 91125 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1993年 / 115卷 / 07期

关键词：

D O I：

10.1021/ja00060a004

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The two peptides distamycin A (D)and 1-methylimidazole-2-carboxamide-netropsin (2-ImN) simultaneously bind the five-base-pair sequence 5'-TGTTA-3'. Footprinting experiments indicate that both molecules are necessary for binding to this sequence while affinity cleaving data define the orientations with which each peptide binds to this sequence. The footprinting and affinity cleaving data are consistent with a model wherein the two peptides bind as a side-by-side antiparallel heterodimer in the minor groove of double-helical DNA.

引用

页码：2572 / 2576

页数：5

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