EFFECT OF SPECIFIC ENZYME-INHIBITORS ON REPLICATION, TOTAL GENOME DNA-REPAIR AND ON GENE-SPECIFIC DNA-REPAIR AFTER UV IRRADIATION IN CHO CELLS

被引：24

作者：

JONES, JC

STEVNSNER, T

MATTERN, MR

BOHR, VA

机构：

[1] NCI,DIV CANC TREATMENT,MOLEC PHARMACOL LAB,BETHESDA,MD 20892

[2] SMITH KLINE BEECHAM PHARMACEUT,DEPT BIOMOLEC DISCOVERY,KING OF PRUSSIA,PA 19406

来源：

MUTATION RESEARCH | 1991年 / 255卷 / 02期

关键词：

ENZYME INHIBITORS; EFFECT ON REPLICATION; REPLICATION; INHIBITION; TOTAL GENOME DNA REPAIR; INHIBITION OF DNA REPAIR; GENE-SPECIFIC DNA REPAIR; DIHYDROFOLATE REDUCTASE GENE; APHIDICOLIN; DNA POLYMERASE; RIBONUCLEOTIDE REDUCTASE; TOPOISOMERASE;

D O I：

10.1016/0921-8777(91)90049-U

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have studied the effect of some specific enzyme inhibitors on DNA repair and replication after UV damage in Chinese hamster ovary cells. The DNA repair was studied at the level of the average, overall genome and also in the active dihydrofolate reductase gene. Replication was measured in the overall genome. We tested inhibitors of DNA polymerase alpha and delta (aphidicolin), of poly(ADPr) polymerase (3-aminobenzamide), of ribonucleotide reductase (hydroxyurea), of topoisomerase I (camptothecin), and of topoisomerase II (merbarone, VP-16). In addition, we tested the effect of the potential topoisomerase I activator, beta-lapachone. All of these compounds inhibited genome replication and all topoisomerase inhibitors affected the overall genome repair; beta-lapachone stimulated it. None of these compounds had any effect on the gene-specific repair.

引用

页码：155 / 162

页数：8

共 36 条

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