SIMULTANEOUS DETERMINATION OF GAMMA-AMINOBUTYRIC-ACID AND POLYAMINES BY ORTHO-PHTHALALDEHYDE BETA-MERCAPTOETHANOL PRECOLUMN DERIVATIZATION AND GRADIENT ELUTION LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

被引：15
作者：
ZAMBONIN, PG
GUERRIERI, A
ROTUNNO, T
PALMISANO, F
机构：
来源：
ANALYTICA CHIMICA ACTA | 1991年 / 251卷 / 1-2期
关键词：
LIQUID CHROMATOGRAPHY; AMINOBUTYRIC ACID; BIOLOGICAL SAMPLES; CADAVERINE; POLYAMINES; PUTRESCINE; SPERMIDINE; SPERMINE;
D O I：
10.1016/0003-2670(91)87121-M
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for the simultaneous determination of gamma-aminobutyric acid, putrescine, spermine, spermidine and cadaverine is described. A precolumn derivatization procedure utilizing omicron-phthalaldehyde-beta-mercaptoethanol is employed to form electroactive isoindole derivatives, which are separated by reversed-phase gradient elution chromatography and detected amperometrically (+ 650 mV vs. Ag/AgCl) at a glassy carbon electrode. By careful optimization of the experimental conditions, the baseline drift during the gradient run could be contained within 0.5 nA. On-column detection limits are typically in the low pmol range and linear responses in the 5-200 pmol injected range. The procedure has been applied to rat brain, kidney and liver tissues after homogenization with perchloric acid.
引用
收藏
页码：101 / 107
页数：7
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