SIMPLE PURIFICATION OF ACETONITRILE FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY (HPLC) OF PEPTIDES AND PROTEINS

被引：0

作者：

BRAUNITZER, G

RUCKNAGEL, P

OBERTHUR, W

机构：

来源：

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE | 1982年 / 363卷 / 05期

关键词：

D O I：

10.1515/bchm2.1982.363.1.485

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method of purifying commercially available acetonitrile by column chromatography over aluminium oxide (neutral) is reported. The absorption at 220 or 230 nm is decreased to values (A = 0.03) which provide a constant baseline in high performance liquid chromatography: the analyzes of proteins and peptides are easier.

引用

页码：485 / 486

页数：2

共 3 条

[1] PREPARATIVE SEPARATION OF THE TRYPTIC HYDROLYSATE OF A PROTEIN BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY - THE PRIMARY STRUCTURE OF A MONOCLONAL L-CHAIN OF KAPPA-TYPE, SUBGROUP-I (BENCE-JONES PROTEIN WES) [J].