OPTIMIZATION OF THE SENSITIVITY OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN THE DETECTION OF HETEROCYCLIC AROMATIC AMINE MUTAGENS

被引：73

作者：

GROSS, GA ^{[1
]}

GRUTER, A ^{[1
]}

HEYLAND, S ^{[1
]}

机构：

[1] VITORECO LTD,CH-8310 KEMPTTAL,SWITZERLAND

来源：

FOOD AND CHEMICAL TOXICOLOGY | 1992年 / 30卷 / 06期

关键词：

D O I：

10.1016/0278-6915(92)90100-Y

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Solid-phase extraction with the weak cation-exchange resin Fractogel TSK CM650(S) (TSK CM) was used to optimize the sensitivity and chromatographic resolution by HPLC of the mutagenic heterocyclic aromatic amines MeIQx, 4,8-DiMeIQx, IQ, MeIQ, PhIP, Trp-P-1, Trp-P-2, amino-alpha-carboline and the co-mutagens norharman and harman. The clean-up using cation exchange was applied after purification on Extrelut and propylsulphonic acid-silica gel cartridges (PRS tandem purification). Only the clean-up with TSK CM enabled low detection limits of the mutagenic compounds to be achieved (in the order of ng heterocyclic amines/g experimentally overheated process-flavour sample).

引用

页码：491 / 498

页数：8

共 14 条

[1]

BLEVINS DD, 1985, SORBENT EXTRACTION T, P4

[2]

DORNER WG, 1983, FACHZEITSCHRIFT LABO, V27, P380

[3] SIMPLE METHODS FOR QUANTIFYING MUTAGENIC HETEROCYCLIC AROMATIC-AMINES IN FOOD-PRODUCTS [J].