STRUCTURE AND EXPRESSION OF A SUGARCANE GENE ENCODING A HOUSEKEEPING PHOSPHOENOLPYRUVATE CARBOXYLASE

被引：19

作者：

ALBERT, HA ^{[1
]}

MARTIN, T ^{[1
]}

SUN, SSM ^{[1
]}

机构：

[1] UNIV HAWAII,DEPT PLANT MOLEC PHYSIOL,3190 MAILE WAY,HONOLULU,HI 96822

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 20卷 / 04期

关键词：

PEP CARBOXYLASE; HOUSEKEEPING GENE; GENE EXPRESSION; GENE STRUCTURE; LIGHT-MEDIATED ACTIVATION; SACCHARUM;

D O I：

10.1007/BF00046451

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from -1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A low-level, essentially constitutive pattern of expression, amino acid sequence similarities to other 'housekeeping' PEPC enzymes, and the absence of DNA sequence elements conserved in the upstream region of maize and sorghum C-4-specific PEPC genes indicate that SCPEPCD1 encodes a housekeeping PEPC. Despite this, a motif proposed to act as a phosphorylation site in light-mediated activation of photosynthetic PEPC enzymes [10] is present in the SCPEPCD1 protein; evidence is presented for the presence of this site in other housekeeping PEPC proteins.

引用

页码：663 / 671

页数：9

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