COMPARATIVE-STUDY OF A REGULAR PROTEIN LAYER BY SCANNING TUNNELING MICROSCOPY AND TRANSMISSION ELECTRON-MICROSCOPY

被引：25

作者：

AMREIN, M ^{[1
]}

WANG, Z ^{[1
]}

GUCKENBERGER, R ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

来源：

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B | 1991年 / 9卷 / 02期

关键词：

D O I：

10.1116/1.585220

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Scanning tunneling microscopy (STM) imaging has been performed on the HPI layer (hexagonally packed intermediate layer), freeze-dried and coated with Pt-Ir-C. The HPI layer is a highly corrugated regular protein monolayer, found outermost in the cell wall of the bacterium Deinococcus radiodurans. The average structure of each of the two surface aspects of HPI layer has been determined from STM data using correlation averaging. The signal-to-noise ratio of the raw data obtained from STM of the HPI layer is in the range of 1.5 to 2, values much higher than those usually encountered in data obtained by transmission electron microscopy (TEM). In order to discuss reliability and accuracy of STM imaging, the reliefs obtained by STM are compared to reconstructed reliefs derived from TEM micrographs of freeze-dried and metal-coated specimens.

引用

页码：1276 / 1281

页数：6

共 17 条

[1] SCANNING TUNNELING MICROSCOPY OF RECA-DNA COMPLEXES COATED WITH A CONDUCTING FILM [J].