SOLUBILIZATION OF CALFSKIN COLLAGEN IN CITRATE BUFFER WITH USE OF AUTOMATED EQUIPMENT

被引：5

作者：

KAHN, LD

WITNAUER, LP

机构：

[1] Eastern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture, Philadelphia, Pennsylvania

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 1969年 / 13卷 / 01期

关键词：

D O I：

10.1002/app.1969.070130115

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubilization of 6.95% of a calfskin corium as monomeric collagen in citrate buffer at pH 3.44 and ionic strength of 0.44 and a temperature of 8°C. It is shown that this is the limit of solubilization for undenatured collagen in this buffer. Copyright © 1969 John Wiley & Sons, Inc.

引用

页码：141 / &

共 13 条

[1]

BELISARIO TM, 1965, J AMER LEATHER CHEM, V60, P393

[2]

CHAPMAN JA, 1966, S INT BIOCHEMIE PHYS, P265

[3]

COMPTE P, 1966, S INT BIOCHEMIE P ED, P265

[4]

COMTE P, 1963, B ASSOC FRANC INGRS, V25, P141

[5]

COMTE P, 1963, B ASSOC FRANC INGRS, V25, P99

[6] STRUCTURAL CHANGES IN COLLAGEN - ACTION OF ALKALIS AND ACIDS IN THE CONVERSION OF COLLAGEN INTO EUCOLLAGEN [J].

COURTS, A .

BIOCHEMICAL JOURNAL, 1960, 74 :238-247

[7] PARTICLE SIZE AND SHAPE IN A CITRATE EXTRACT OF ICHTHYOCOL [J].

GALLOP, PM .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1955, 54 (02) :486-500

[8] CHONDROITIN SULPHURIC ACID AS A FACTOR IN THE STABILITY OF TENDON [J].

JACKSON, DS .

BIOCHEMICAL JOURNAL, 1953, 54 (05) :638-641

[9]

MAZUROV VI, 1959, BIOCHEMISTRY-MOSCOW+, V24, P28

[10]

NISHIHARA T, 1963, Patent No. 9295

← 1 2 →