ACTIVITY OF ENZYMES INVOLVED IN CAPSAICIN BIOSYNTHESIS IN CALLUS-TISSUE AND FRUITS OF CHILI-PEPPER (CAPSICUM-ANNUUM-L)

被引：85
作者：
OCHOAALEJO, N
GOMEZPERALTA, JE
机构：
[1] Centro de Investigación y de Estudios Avanzados del IPN-Unidad Irapuato, Irapuato, Guanajuato, 36500
来源：
JOURNAL OF PLANT PHYSIOLOGY | 1993年 / 141卷 / 02期
关键词：
CAPSAICINOID PATHWAY; CAPSAICIN BIOSYNTHESIS; CALLUS CULTURES; CHILI PEPPER; CAPSICUM-ANNUUM;
D O I：
10.1016/S0176-1617(11)80751-0
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In order to examine the functionality of the capsaicinoid biosynthetic pathway in callus cultures of chili pepper (Capsicum annuum L.), we investigated the enzyme activity of phenylalanine ammonia-lyase (PAL), cinnamic acid-4-hydroxylase (CA4H), p-coumaric acid-3-hydroxylase (CA3H), caffeic acid-O-methyltransferase (CAOMT) and capsaicinoid synthetase (CS). These enzymes were also determined in developing and mature fruits. The maximum activities of PAL, CA4H and CA3H in callus cultures were similar to those of chili pepper fruits, but the values of CAOMT and CS were 6 times lower than those of fruits. The significance of these data in relation to capsaicin accumulation is discussed.
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页码：147 / 152
页数：6
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