ACTIVITY OF ENZYMES INVOLVED IN CAPSAICIN BIOSYNTHESIS IN CALLUS-TISSUE AND FRUITS OF CHILI-PEPPER (CAPSICUM-ANNUUM-L)

被引：87

作者：

OCHOAALEJO, N

GOMEZPERALTA, JE

机构：

[1] Centro de Investigación y de Estudios Avanzados del IPN-Unidad Irapuato, Irapuato, Guanajuato, 36500

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1993年 / 141卷 / 02期

关键词：

CAPSAICINOID PATHWAY; CAPSAICIN BIOSYNTHESIS; CALLUS CULTURES; CHILI PEPPER; CAPSICUM-ANNUUM;

D O I：

10.1016/S0176-1617(11)80751-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In order to examine the functionality of the capsaicinoid biosynthetic pathway in callus cultures of chili pepper (Capsicum annuum L.), we investigated the enzyme activity of phenylalanine ammonia-lyase (PAL), cinnamic acid-4-hydroxylase (CA4H), p-coumaric acid-3-hydroxylase (CA3H), caffeic acid-O-methyltransferase (CAOMT) and capsaicinoid synthetase (CS). These enzymes were also determined in developing and mature fruits. The maximum activities of PAL, CA4H and CA3H in callus cultures were similar to those of chili pepper fruits, but the values of CAOMT and CS were 6 times lower than those of fruits. The significance of these data in relation to capsaicin accumulation is discussed.

引用

收藏

页码：147 / 152

页数：6

相关论文

共 22 条

[1] CONSTITUTION AND BIOSYNTHESIS OF CAPSAICIN [J].

BENNETT, DJ ;

KIRBY, GW .

JOURNAL OF THE CHEMICAL SOCIETY C-ORGANIC, 1968, (04) :442-&

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] FORMATION AND METABOLISM OF PUNGENT PRINCIPLE OF CAPSICUM FRUITS .7. ENZYMATIC FORMATION OF CAPSAICINOID FROM VANILLYLAMINE AND ISO-TYPE FATTY-ACIDS BY CELL-FREE-EXTRACTS OF CAPSICUM-ANNUUM VAR ANNUUM CV KARAYATSUBUSA [J].

FUJIWAKE, H ;

SUZUKI, T ;

OKA, S ;

IWAI, K .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1980, 44 (12) :2907-2912

[4]

FUJIWAKE H, 1980, PLANT CELL PHYSIOL, V21, P1023

[5] THE ACCUMULATION OF PHENYLPROPANOID AND CAPSAICINOID COMPOUNDS IN CELL-CULTURES AND WHOLE FRUIT OF THE CHILIC PEPPER, CAPSICUM-FRUTESCENS MILL [J].

HALL, RD ;

HOLDEN, MA ;

YEOMAN, MM .

PLANT CELL TISSUE AND ORGAN CULTURE, 1987, 8 (02) :163-176

[6]

HALL RD, 1991, PLANTA, V185, P72, DOI 10.1007/BF00194517

[7] INHIBITION OF FLAVONOID BIOSYNTHESIS BY GIBBERELLIC-ACID IN CELL-SUSPENSION CULTURES OF DAUCUS-CAROTA L [J].

HINDERER, W ;

PETERSEN, M ;

SEITZ, HU .

PLANTA, 1984, 160 (06) :544-549

[8] FORMATION AND ACCUMULATION OF PUNGENT PRINCIPLE OF HOT PEPPER FRUITS, CAPSAICIN AND ITS ANALOGS, IN CAPSICUM-ANNUUN VAR ANNUUN CV KARAYATSUBUSA AT DIFFERENT GROWTH-STAGES AFTER FLOWERING [J].

IWAI, K ;

SUZUKI, T ;

FUJIWAKE, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1979, 43 (12) :2493-2498

[9] FORMATION AND METABOLISM OF PUNGENT PRINCIPLES OF CAPSICUM FRUITS .3. INTRACELLULAR-LOCALIZATION OF CAPSAICINOID SYNTHESIZING ENZYME IN SWEET PEPPER FRUITS [J].

IWAI, K ;

LEE, KR ;

KOBASHI, M ;

SUZUKI, T ;

OKA, S .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1978, 42 (01) :201-202

[10] INVITRO CAPSAICIN PRODUCTION BY IMMOBILIZED CELLS AND PLACENTAL TISSUES OF CAPSICUM-ANNUUM-L GROWN IN LIQUID-MEDIUM [J].

JOHNSON, TS ;

RAVISHANKAR, GA ;

VENKATARAMAN, LV .

PLANT SCIENCE, 1990, 70 (02) :223-229