The content and distribution of amathamide alkaloids within single colonies of the bryozoan Amathia wilsoni (Ctenostomata) varied depending on the location in the colony. Three colonies in all, collected from the same site at the same time, were analyzed and gave very similar results. The outermost, more exposed, tips of the colony had an alkaloid content of nearly 9 % of dry weight, while basal parts were apparently devoid of alkaloids. Samples taken midway between tips and base yielded intermediate concentrations of about 1 %. Very little variation in the proportions of individual amathamides A, B, C, E occurred between exposed tips of the colonies. However, some differences in ratios were found between tips from exposed and more protected regions.