GENOMIC STRUCTURE OF THE GENE FOR COPPER/ZINC-SUPEROXIDE DISMUTASE IN RICE

被引:23
|
作者
SAKAMOTO, A [1 ]
OKUMURA, T [1 ]
OHSUGA, H [1 ]
TANAKA, K [1 ]
机构
[1] KYOTO PREFECTURAL UNIV,COLL AGR,DEPT BIOCHEM,KYOTO 606,JAPAN
关键词
ACTIVE OXYGEN; EXON/INTRON; GENOMIC DNA SEQUENCE; SOD GENE; SUPEROXIDE DISMUTASE; RICE (ORYZA-SATIVA L);
D O I
10.1016/0014-5793(92)81244-G
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated and determined the nucleotide sequence of the structural gene (sodB) coding for one of the copper/zinc-superoxide dismutase (Cu/Zn-SOD) isozymes from rice plants (Oryza sativa L.). The sodB gene is split into 8 exons spread about 2 kb of the chromosomal DNA, with the coding sequence beginning in the 2nd and ending in the 8th. Although none of sodB introns are inserted into similar positions in Cu/Zn-SOD genes from other sources, four of six introns in the protein-coding region are located at discriminating positions within structural domains of the protein. Genomic Southern analysis indicated that cytosolic Cu/Zn-SOD is coded for by 2-3 genes in the rice genome.
引用
收藏
页码:185 / 189
页数:5
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