HYBRID BIOSENSOR FOR THE DETERMINATION OF LACTOSE

被引：20

作者：

SVORC, J ^{[1
]}

MIERTUS, S ^{[1
]}

BARLIKOVA, A ^{[1
]}

机构：

[1] SLOVAK UNIV TECHNOL BRATISLAVA, DEPT ANALYT CHEM, CS-81237 BRATISLAVA, CZECHOSLOVAKIA

来源：

ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 15期

关键词：

D O I：

10.1021/ac00214a018

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Genetically manipulated bacteria Escherichia coll K-12 recombinant PQ-37 and glucose oxidase (EC 1.1.3.4) were used for the construction of a hybrid lactose sensor because of the hyperproduction of β-galactosidase (EC 3.2.1.23) by E. coll effected by a genotoxlc agent. The blocatalytic layer was prepared by coimmobilization of the E. coll cells with glucose oxidase on the nylon network via giutardiaidehyde and fixed to the Clark oxygen electrode. The Influence of pH, temperature, and concentration of activators of β-galactosidase on the sensor response was tested. Analyses of milk products were completed without any special pretreatment of the samples. The contents of lactose determined by hybrid sensor agree with conventional photometric measurements. Standard relative deviation is less than 3% for all samples. The half-life of operational stability is 30 days. © 1990, American Chemical Society. All rights reserved.

引用

页码：1628 / 1631

页数：4

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