PROPERTIES OF LEUKOKININOGEN ISOLATED FROM HUMAN NEOPLASTIC ASCITES

被引：14

作者：

ROFFMAN, S

GREENBAUM, LM

机构：

[1] Department of Pharmacology, College of Physicians and Surgeons, Columbia University, New York

来源：

BIOCHEMICAL PHARMACOLOGY | 1979年 / 28卷 / 07期

关键词：

D O I：

10.1016/0006-2952(79)90301-0

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Leukokininogen, the protein precursor of the vasoactive polypeptide leukokinin-H, has been isolated from human ovarian carcinoma ascites. A homogeneous preparation was achieved after a procedure consisting of ammonium sulfate fractionation, gel filtration with Sephadex G-200, DEAE-Sepharose ion exchange chromatography and preparative isoelectric focusing. Leukokininogen has an isoelectric pH of 4.56 and a molecular weight of about 41,000 daltons, determined by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate. Kinin-like activity was generated from leukokininogen by incubation of the protein with the leukokininogenase from murine ascites tumor cells and with trypsin. Human plasma kallikrein does not release kinin activity from leukokininogen. The amino acid composition and immunological properties of leukokininogen demonstrate that leukokininogen is distinct from plasma bradykininogens. © 1979.

引用

页码：1043 / 1050

页数：8

共 27 条

[1] STUDIES ON LEUKOKININS .2. STUDIES ON FORMATION, PARTIAL AMINO-ACID SEQUENCE AND CHEMICAL PROPERTIES OF LEUKOKININS-M AND LEUKOKININS-PMN [J].