PHOSPHATIDYLINOSITOL 3-KINASE REGULATION OF FLUID-PHASE ENDOCYTOSIS

被引：71

作者：

CLAGUE, MJ

THORPE, C

JONES, AT

机构：

[1] Physiological Laboratory, University of Liverpool, Liverpool, L69 3BX, Crown St

来源：

FEBS LETTERS | 1995年 / 367卷 / 03期

基金：

英国惠康基金;

关键词：

PI; 3-KINASE; ENDOCYTOSIS; WORTMANNIN; RECEPTOR DOWN-REGULATION; DYNAMIN;

D O I：

10.1016/0014-5793(95)00576-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Endocytosis of the fluid phase marker, horse radish peroxidase, into baby hamster kidney cells is inhibited by treatment of cells with the fungal metabolite wortmannin, The IC50 of approximately 5 nM is consistent with the well-described action of wortmannin upon phosphatidylinositol (PI) 3-kinase, Analysis of the kinetics of uptake indicates a > 50% decrease in the initial rate of marker internalisation, a concomitant decrease in the volume of the early endosome and an increased efficiency of recycling of that marker which is internalised, As PI 3-kinase binds to activated growth factor receptors our data suggest that receptor activation can be coupled to receptor internalisation (down regulation) by localising PI 3-kinase stimulation of endocytosis.

引用

页码：272 / 274

页数：3

共 22 条

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