OPTIMIZATION OF THE SISTROM CULTURE-MEDIUM FOR LARGE-SCALE BATCH CULTIVATION OF RHODOSPIRILLUM-RUBRUM UNDER SEMIAEROBIC CONDITIONS WITH MAXIMAL YIELD OF PHOTOSYNTHETIC MEMBRANES

被引:39
|
作者
GHOSH, R [1 ]
HARDMEYER, A [1 ]
THOENEN, I [1 ]
BACHOFEN, R [1 ]
机构
[1] UNIV ZURICH,INST PLANT BIOL,CH-8008 ZURICH,SWITZERLAND
关键词
D O I
10.1128/AEM.60.5.1698-1700.1994
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The defined medium A of W. R. Sistrom (W. R. Sistrom, J. Gen. Microbiol. 22:77-85, 1960) has been modified to allow the growth of Rhodospirillum rubrum in large-scale batch cultures under dark, semiaerobic conditions. The simultaneous use of two substrates, NH4-succinate (46 mM) and fructose (0.3%), which are utilized in aerobic and fermentative metabolism, respectively, leads to very high cell densities with a maximal yield of photosynthetic membranes.
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页码:1698 / 1700
页数:3
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