ULTRASTRUCTURAL-LOCALIZATION OF PEMPHIGUS VULGARIS AND PEMPHIGUS FOLIACEUS ANTIGENS IN CULTURED HUMAN SQUAMOUS CARCINOMA-CELLS

被引：26
作者：
AKIYAMA, M
HASHIMOTO, T
SUGIURA, M
NISHIKAWA, T
机构：
[1] KEIO UNIV,SCH MED,DEPT DERMATOL,TOKYO 108,JAPAN
[2] SHIMIZU CITY HOSP,DEPT DERMATOL,SHIZUOKA,JAPAN
来源：
BRITISH JOURNAL OF DERMATOLOGY | 1991年 / 125卷 / 03期
关键词：
D O I：
10.1111/j.1365-2133.1991.tb14746.x
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
The ultrastructural localization of the pemphigus vulgaris (PV) and pemphigus foliaceus (PF) antigens in cultured human squamous carcinoma cells was observed using immunogold electron microscopy. Both the PV and PF autoantibodies bound only to the extracellular portion of the desmosomal structures. After incubation at 37-degrees-C, the PV antigen-antibody complexes were observed within the cultured cells. PV and PF antigen expression was markedly reduced when the cells were cultured in medium with a low Ca2+ concentration.
引用
收藏
页码：233 / 237
页数：5
相关论文
共 26 条
[1]   SIMULTANEOUS DETECTION OF MEMBRANE MARKERS WITH MONOCLONAL-ANTIBODIES AND PEROXIDATIC ACTIVITIES IN LEUKEMIA - ULTRASTRUCTURAL ANALYSIS USING A NEW METHOD OF FIXATION PRESERVING THE PLATELET PEROXIDASE [J].
BRETONGORIUS, J ;
VANHAEKE, D ;
PRYZWANSKY, KB ;
GUICHARD, J ;
TABILIO, A ;
VAINCHENKER, W ;
CARMEL, R .
BRITISH JOURNAL OF HAEMATOLOGY, 1984, 58 (03) :447-458
[2]  
CITOVSKY V, 1983, J INVEST DERMATOL, V80, P366
[3]   HUMAN AUTOANTIBODIES AGAINST A DESMOSOMAL PROTEIN COMPLEX WITH A CALCIUM-SENSITIVE EPITOPE ARE CHARACTERISTIC OF PEMPHIGUS FOLIACEUS PATIENTS [J].
EYRE, RW ;
STANLEY, JR .
JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 165 (06) :1719-1724
[4]   IDENTIFICATION OF PEMPHIGUS VULGARIS ANTIGEN EXTRACTED FROM NORMAL HUMAN-EPIDERMIS AND COMPARISON WITH PEMPHIGUS FOLIACEUS ANTIGEN [J].
EYRE, RW ;
STANLEY, JR .
JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (03) :807-812
[5]   DESMOSOMAL ANTIGENS ARE NOT RECOGNIZED BY THE MAJORITY OF PEMPHIGUS AUTOIMMUNE SERA [J].
GORBSKY, G ;
COHEN, S ;
STEINBERG, MS .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1983, 80 (06) :475-480
[6]   DETECTION OF PEMPHIGUS VULGARIS AND PEMPHIGUS FOLIACEUS ANTIGENS BY IMMUNOBLOT ANALYSIS USING DIFFERENT ANTIGEN SOURCES [J].
HASHIMOTO, T ;
OGAWA, MM ;
KONOHANA, A ;
NISHIKAWA, T .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1990, 94 (03) :327-331
[7]   EXPERIMENTAL SUPRABASAL BULLA FORMATION IN ORGAN CULTURED HUMAN-SKIN WITH LOW CALCIUM MEDIUM [J].
HASHIMOTO, T .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1988, 90 (04) :501-504
[8]  
HOFFMAN HT, 1990, ARCH OTOLARYNGOL, V116, P299
[9]   INVIVO BINDING-SITE OF PEMPHIGUS VULGARIS ANTIBODIES AND THEIR FATE DURING ACANTHOLYSIS [J].
IWATSUKI, K ;
TAKIGAWA, M ;
IMAIZUMI, S ;
YAMADA, M .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1989, 20 (04) :578-582
[10]   A CELL-SURFACE DESMOSOME-ASSOCIATED COMPONENT - IDENTIFICATION OF A TISSUE-SPECIFIC CELL-ADHESION MOLECULE [J].
JONES, JCR ;
YOKOO, KM ;
GOLDMAN, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (19) :7282-7286
123