PURIFICATION OF MOUSE INTERFERON BY SEQUENTIAL CHROMATOGRAPHY

被引：12

作者：

BRAUDE, IA ^{[1
]}

DECLERCQ, E ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,REGA INST MED RES,DEPT HUMAN BIOL,B-3000 LOUVAIN,BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 172卷 / APR期

关键词：

D O I：

10.1016/S0021-9673(00)90956-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two schemes for the purification of mouse interferon are described, based on the concerted application of various physicochemical and affinity/adsorption column chromatographic techniques. Mouse interferon was purified to a final specific activity of 1.0-8.0 x 108 units/mg when first precipitated with ammonium sulphate and further processed by hydrophobic chromatography and adsorption chromatography on AFFI-Gel 202 and Controlled Pore Glass. It was purified to a final specific activity of 2.5-3.7 x 108 units/mg when first precipitated with ammonium sulphate and further processed by gel filtration with Ultrogel AcA 54, ion-exchange chromatography with Carboxymethyl Bio-Gel Agarose, hydrophobic chromatography with AFFI-Gel 202 and adsorption chromatography with Controlled Pore Glass. © 1979.

引用

页码：207 / 219

页数：13

共 32 条

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