VIRAL INACTIVATION OF VESICULAR STOMATITIS-VIRUS IN NORMAL HUMAN SERUM BY CROSS-LINKED POLYVINYLPYRROLIDONE

Methods for inactivating virus contaminants in serum, cryoprecipitate-poor plasma, and protein concentrates need to be identified. In this study, vesicular stomatitis virus (VSV)-spiked human serum and cryoprecipitate-poor plasma were treated with cross-linked povidone iodine (XLPVPI) at concentrations of 0, 4, 6, 8, and 10 mg/mL up to 120 min at 4 and 24-degrees-C. The activities of virus and relevant proteins were examined. The results indicated that XLPVPI at concentrations that inactivate >5 logs of VSV in serum decreased factor IX and protein C activities by <10% in cryoprecipitate-poor plasma. At concentrations up to 10 mg of XLPVPI/mL, <10% of protein C and factor IX activity was lost after incubation for 5 min at 24-degrees-C. In addition, < 10% loss in protein C and factor IX activity was observed at 4-degrees-C after treatment with less-than-or-equal-to 6 mg of XLPVPI/mL for 20 min. Treatment of human serum with 6 mg of XLPVPI/mL at 4-degrees-C and 8 mg of XLPVPI/mL at 24-degrees-C for 5 min provided inactivation of >5 logs of VSV.