PRODUCTION OF ACID PROTEINASES BY HUMICOLA-LUTEA MYCELIUM IMMOBILIZED IN POLYURETHANE SPONGE

被引：7
作者：
ALEKSIEVA, P
TCHORBANOV, B
机构：
[1] BULGARIAN ACAD SCI,INST ORGAN CHEM,CTR PHYTOCHEM,BU-1040 SOFIA,BULGARIA
[2] BULGARIAN ACAD SCI,INST MICROBIOL,BU-1040 SOFIA,BULGARIA
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 12期
关键词：
acid proteinases; Humicola lutea; immobilization; polyurethane;
D O I：
10.1016/0141-0229(90)90123-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Humicola lutea 120-5 spores were entrapped in polyurethane sponge cubes and were cultivated inside the carrier to form an immobilized mycelium further used for production of acid proteinases in batch mode. A carrier-spore suspension ratio of 10:0.5 (wt) should be used to obtain optimal results. The polyurethane sponge-immobilized mycelium could be applied repeatedly, the enzyme activity secreted during the first 10 cycles being about the same as that produced by free cells. The advantages of immobilizing fungal cells by germinating conidia entrapped inside the supporting material are discussed. © 1990.
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页码：994 / 996
页数：3
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