PHOSPHATE-DEPENDENT MONOCLONAL-ANTIBODIES TO NEUROFILAMENTS AND ALZHEIMER NEUROFIBRILLARY TANGLES RECOGNIZE A SYNTHETIC PHOSPHOPEPTIDE

被引：44

作者：

COLEMAN, MP ^{[1
]}

ANDERTON, BH ^{[1
]}

机构：

[1] INST PSYCHIAT,DEPT NEUROSCI,LONDON SE5 8AF,ENGLAND

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 05期

基金：

英国惠康基金;

关键词：

Alzheimer's disease; Monoclonal antibodies; Neurofibrillary tangles; Neurofilaments; Phosphorylation;

D O I：

10.1111/j.1471-4159.1990.tb01203.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: Five phosphate‐dependent monoclonal antibodies to the neurofilament heavy polypeptide bound strongly to a phosphorylated synthetic peptide, which contains a single Lys‐Ser‐Pro sequence that occurs in human neurofilaments. Three of the antibodies label Alzheimer's disease neurofibrillary tangles and two do not, suggesting that in tangles an epitope similar to the peptide is available to some but not all of the antibodies. In addition, some antibodies were found to be more affected than others by enzymatic dephosphorylation of the antigen, but because all the antibodies bound the same synthetic phosphopeptide they do not bind to mutually exclusive phosphorylation sites. Instead the more phosphate‐dependent antibodies might bind the phosphate group more directly, as suggested by their inhibition by inorganic phosphate and free phosphoserine. Copyright © 1990, Wiley Blackwell. All rights reserved

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页码：1548 / 1555

页数：8

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