3 DISTINCT REGIONS OF CHROMOSOME-6 ARE TARGETS OF LOSS OF HETEROZYGOSITY IN HUMAN OVARIAN CARCINOMAS

We performed mapping studies of allelic deletions on chromosome 6 in 52 ovarian carcinomas using 20 polymorphic markers assigned to various regions of both chromosomal arms. Loss of heterozygosity was seen in 28 (55%) tumors. The allelic losses, however, did not involve the whole chromosome in 18 of those tumors, allowing further localization of the targeted chromosomal region(s). Three different chromosomal regions appeared involved based on the distribution of partial deletions, 2 on the long arm and one on the short arm. One of the 2 regions on the long arm was confined to band 6q27 whereas the other was between the Arg1 and estrogen receptor loci. The targeted region on the short arm was bounded by the D6S89 and D6S248 loci. These results suggest the presence of at least 3 different tumor suppressor genes important for the control of ovarian tumorigenesis on chromosome 6.