OPIATE (ENKEPHALIN) RECEPTORS OF NEURO-BLASTOMA CELLS - OCCURRENCE IN CLUSTERS ON THE CELL-SURFACE

被引：85
作者：
HAZUM, E
CHANG, KJ
CUATRECASAS, P
机构：
[1] Department of Molecular Biology, Wellcome Research Laboratories, Research Triangle Park
来源：
SCIENCE | 1979年 / 206卷 / 4422期
关键词：
D O I：
10.1126/science.227058
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A bioactive, fluorescent derivative of enkephalin, Tyr-D-Ala-Gly-PheLeu- Lys-rhodamine, was used to determine the distribution of opiate receptors in living neuroblastoma cells. The receptors appeared in clusters on the cell surface, and no internalization was detected. No specific fluorescence or clusters were observed in the presence of [D-Ala2, Leu 5]enkephalin or at 4°C, and the clusters were much reduced under ionic conditions (that is, with 100 millimolars sodium) that specifically decrease the binding of opiate agonists. Copyright © 1979 AAAS.
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页码：1077 / 1079
页数：3
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