EXTRACTION, PARTIAL-PURIFICATION AND CHARACTERIZATION OF A BACTERIOCIN (FRAGILICIN) PRODUCED BY A STRAIN OF BACTEROIDES-FRAGILIS ISOLATED FROM CALLITHRIX PENICILLATA

被引：18
作者：
FARIAS, LM
TOTOLA, AH
MIRANDA, CMS
CARVALHO, MAR
DAMASCENO, CAV
TAVARES, CAP
CISALPINO, EO
VIEIRA, EC
机构：
[1] UNIV FED MINAS GERAIS,DEPT MICROBIOL,BR-30161960 BELO HORIZONT,MG,BRAZIL
[2] UNIV FED MINAS GERAIS,INST CIENCIAS BIOL,DEPT BIOQUIM & IMMUNOL,BR-30161960 BELO HORIZONT,MG,BRAZIL
来源：
RESEARCH IN MICROBIOLOGY | 1994年 / 145卷 / 01期
关键词：
BACTERIOCIN; FRAGICILIN; PROTEIN; BACTEROIDES FRAGILIS; ANAEROBIOSIS; CHARACTERIZATION; ECOSYSTEMS; ANTAGONISMS;
D O I：
10.1016/0923-2508(94)90062-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A strain of Bacteroides fragilis, isolated from the marmoset Callithrix penicillata, produced protein(s) with bacteriocin activity (fragilicin). Two active fractions (36 and 150 kDa) were isolated by chromatography. The bacteriocin exhibited iso- and hetero-antagonism. It remained stable between pH 3 and 10 and at 60 degrees C for 24 h. Pronase, trypsin, proteinase K and type VII protease inactivated the bacteriocin, giving evidence of its protein nature.
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页码：9 / 16
页数：8
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