Potential biofilm dispersal by a partially purified keratinase produced by Stenotrophomonas maltophilia strain Kb2

被引：10

作者：

Bhange, Khushboo ^{[1
]}

Chaturvedi, Venkatesh ^{[2
]}

Bhatt, Renu ^{[1
]}

机构：

[1] Guru Ghasidas Vishwavidyalaya, Dept Biotechnol, Bilaspur 495009, Chhattisgarh, India

[2] Banaras Hindu Univ, Sch Biotechnol, Varanasi 221005, Uttar Pradesh, India

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2015年 / 4卷 / 04期

关键词：

Stenotrophomonas maltophila Kb2; Keratinase; Biofilm dispersal;

D O I：

10.1016/j.bcab.2015.10.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Biofilm dispersal and antibiofilm activity of the keratinase produced by Stenotrophomonas maltophilia Kb2 was studied against pathogenic bacteria Staphylococcus aureus MTCC-96 and Escherichia coli MTCC-739. The quantitative estimation of biofilm dispersal showed a positive correlation with the concentration of keratinase and biofilm dispersal in both the strains. The dispersal was further confirmed by the microscopic observation of keratinase treated biofilm. Among the two isolates, biofilm dispersal activity of keratinase was more pronounced in S. aureus as compared to E. coli. Inhibition of biofilm formation was observed in both the strains in presence of keratinase and a dose dependent relationship between keratinase concentration and antibiofilm activity was observed. Time course study revealed that with the increasing time duration antibiofilm and biofilm dispersal activity of keratinase decreased. The study would open new avenues in medical field for the removal of biofilms from obstructing indwelling catheters and other medical devices. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：801 / 805

页数：5

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