MUTATIONAL ANALYSIS OF A PLANT HEAT-SHOCK ELEMENT

被引：47
作者：
BARROS, MD
CZARNECKA, E
GURLEY, WB
机构：
[1] Department of Microbiology and Cell Science, University of Florida, Gainesville, 32611, FL
来源：
PLANT MOLECULAR BIOLOGY | 1992年 / 19卷 / 04期
关键词：
HSE; PROMOTER; SOYBEAN; TRANSCRIPTION;
D O I：
10.1007/BF00026792
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A total of 32 mutations were generated within the TATA-proximal site 1 (-72 to -47) of soybean heat shock gene Gmhsp17.5E in order to functionally define the optimal configuration of sequences within the heat shock element (HSE). Mutants were tested in vivo utilizing sunflower tumors transformed by a T-DNA based vector. Promoter activity was determined by S1 nuclease hybrid protection analysis of tumor transcripts. A total of five repeats (5'-nGAAn-3' or 5'-nTTCn-3') which comprise the HSE at site 1 were required for full transcription induction by heat stress. Analysis of non-conserved bases flanking the central trinucleotide block indicated that 5'-aGAAg'-3' is the optimum sequence for the 5 bp repeat.
引用
收藏
页码：665 / 675
页数：11
相关论文
共 41 条
[1]   KEY FEATURES OF HEAT-SHOCK REGULATORY ELEMENTS [J].
AMIN, J ;
ANANTHAN, J ;
VOELLMY, R .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (09) :3761-3769
[2]   ORGANIZATION OF THE DROSOPHILA-MELANOGASTER HSP70 HEAT-SHOCK REGULATION UNIT [J].
AMIN, J ;
MESTRIL, R ;
SCHILLER, P ;
DREANO, M ;
VOELLMY, R .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (03) :1055-1062
[3]   SCREENING GAMMAGT RECOMBINANT CLONES BY HYBRIDIZATION TO SINGLE PLAQUES INSITU [J].
BENTON, WD ;
DAVIS, RW .
SCIENCE, 1977, 196 (4286) :180-182
[4]   FUNCTIONAL DOMAINS OF A T-DNA PROMOTER ACTIVE IN CROWN GALL TUMORS [J].
BRUCE, WB ;
GURLEY, WB .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (01) :59-67
[5]   AGROBACTERIUM-TUMEFACIENS DNA AND PS8 BACTERIOPHAGE DNA NOT DETECTED IN CROWN GALL TUMORS [J].
CHILTON, MD ;
CURRIER, TC ;
FARRAND, SK ;
BENDICH, AJ ;
GORDON, MP ;
NESTER, EW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (09) :3672-3676
[6]   MOLECULAR-CLONING AND EXPRESSION OF A HEXAMERIC DROSOPHILA HEAT-SHOCK FACTOR SUBJECT TO NEGATIVE REGULATION [J].
CLOS, J ;
WESTWOOD, JT ;
BECKER, PB ;
WILSON, S ;
LAMBERT, K ;
WU, C .
CELL, 1990, 63 (05) :1085-1097
[7]   REGULATORY DOMAINS OF THE GMHSP 17.5-E HEAT-SHOCK PROMOTER OF SOYBEAN [J].
CZARNECKA, E ;
KEY, JL ;
GURLEY, WB .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (08) :3457-3463
[8]   COMPARATIVE-ANALYSIS OF PHYSICAL STRESS RESPONSES IN SOYBEAN SEEDLINGS USING CLONED HEAT-SHOCK CDNAS [J].
CZARNECKA, E ;
EDELMAN, L ;
SCHOFFL, F ;
KEY, JL .
PLANT MOLECULAR BIOLOGY, 1984, 3 (01) :45-58
[9]   INVITRO INTERACTION OF NUCLEAR PROTEINS WITH THE PROMOTER OF SOYBEAN HEAT-SHOCK GENE GMHSP17.5E [J].
CZARNECKA, E ;
FOX, PC ;
GURLEY, WB .
PLANT PHYSIOLOGY, 1990, 94 (03) :935-943
[10]   DNA-SEQUENCE AND TRANSCRIPT MAPPING OF A SOYBEAN GENE ENCODING A SMALL HEAT-SHOCK PROTEIN [J].
CZARNECKA, E ;
GURLEY, WB ;
NAGAO, RT ;
MOSQUERA, LA ;
KEY, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (11) :3726-3730
12345