PREPARATION OF TRITIATED LIPOPOLYSACCHARIDES FROM ESCHERICHIA-COLI K12

被引：1

作者：

PEBORDE, JP ^{[1
]}

SAMAIN, D ^{[1
]}

机构：

[1] UNIV PAUL SABATIER,INSA,CTR BIOTECHNOL & MICROBIOL,126 AV RANGUEIL,F-31077 TOULOUSE,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1033卷 / 02期

关键词：

(E. coli K12); Lipopolysaccharide; Radiolabeling;

D O I：

10.1016/0304-4165(90)90014-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Triated lipopolysaccharide (LPS) from E. coli K12 was prepared by coupling [3H]ethanolamine to the LPS core residue ketodeoxyoctonate (KDO) via activation of its carboxylic function with N-hydroxysuccinimide or N-hydroxysulfosuccinimide. Specific activities of 1.5 μCi/mg and 9 μCi/mg were obtained, respectively. Experiments comparing the activity of native and derivatized LPS suggested that the preparation of the radiolabelled LPS did not not alter the structural properties of E. coli K12 LPS. This probe will be useful for studying the interactions between LPS and proteins. © 1990.

引用

页码：207 / 209

页数：3

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