HIGH-SPEED SEPARATIONS OF DNA SEQUENCING REACTIONS BY CAPILLARY ELECTROPHORESIS

被引：231

作者：

DROSSMAN, H ^{[1
]}

LUCKEY, JA ^{[1
]}

KOSTICHKA, AJ ^{[1
]}

DCUNHA, J ^{[1
]}

SMITH, LM ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706

来源：

ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 09期

关键词：

D O I：

10.1021/ac00208a003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fluorescently labeled DNA fragments generated In enzymatic sequencing reactions are rapidly separated by capillary gel electrophoresis and detected at attomole levels within the gel-filled capillary. The application of this technology to automated DNA sequence analysis may permit the development of a second generation automated sequencer capable of efficient and cost-effective sequence analysis on the genomic scale. © 1990 American Chemical Society. © 1990, American Chemical Society. All rights reserved.

引用

页码：900 / 903

页数：4

共 19 条

[1]

BENTE PF, 1988, Patent No. 272925

[2]

BENTE PF, 1836, Patent No. 946568

[3] SUBATTOMOLE AMINO-ACID ANALYSIS BY CAPILLARY ZONE ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE [J].