USE OF LIQUID SCINTILLATION SPECTROMETER FOR DETERMINING ADENOSINE TRIPHOSPHATE BY LUCIFERASE ENZYME

被引：956
作者：
STANLEY, PE
WILLIAMS, SG
机构：
[1] Department of Agricultural Biochemistry, Waite Agricultural Research Institute, The University of Adelaide, SA
来源：
ANALYTICAL BIOCHEMISTRY | 1969年 / 29卷 / 03期
关键词：
D O I：
10.1016/0003-2697(69)90323-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple, economical method is described for determining ATP over the range 10-9 and 10-12 mole, by the firefly luciferin-luciferase system, using any liquid scintillation spectrometer of suitable performance. This procedure has been employed to determine ATP in perchloric acid extracts of biological material. A critical appraisal is made of the factors affecting the liquid scintillation spectrometer for this assay. © 1969.
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页码：381 / &
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